Development of Blood grouping Hybridoma cell line to increase the secretion of monoclonal Antibodies (mAbs) for diagnostic/therapeutic use.

1 year ago

A seminar by Dr Asokan Chinnasamy highlights the role of blood grouping Hybridoma cell line on increasing the secretion of monoclonal anitobodies (mAbs) for diagnostic/therapeutic purposes.

Dr Chinnasamy, from the School of Medical Sciences, gave a presentation on his paper to the UKH staff.

Biopolymer membrane was prepared using two oppositely charged natural polymers. The biopolymer membrane was used for the encapsulation of Hybridoma Anti-D cell line to produce monoclonal antibodies (mAbs). Soy Hydrolysate is one of the supplement and replacement of Fetal calf serum for the growth of the mammalian cells and secretion of the biological products. It contains low molecular weight nutrients, peptides and free amino acids which are essential for cell growth.

Morphologies of beads and the cell growth were observed in inverted Microscope. Soy Hydrolysate was used as animal component free supplement with the different concentrations of 0.1%, 0.3%, 0.5% and 1% with RPMI 1640 medium to enhance the cell growth and increase the monoclonal antibodies secretion. The effect of Soy Hydrolysate is studied by the cell growth and monoclonal antibodies secretion. The Increased secretion of monoclonal antibodies is confirmed by Avidity test, Titer analysis, total protein estimation and size exclusion chromatography analysis.

The present study concludes that increase the yield of diagnosis/therapeutic monoclonal antibodies could be maximized by the encapsulation method. Thus, in the optimization of monoclonal antibodies in cell culture and their increased secretion, additives like Soy Hydrolysate plays a significant role. 

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